Clcn3 Antibody
Catalogue Number: ASM10242-A680-ABG
ASM10242-A680 Image
ASM10242-A680 Image
ASM10242-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Clcn3 Monoclonal Antibody, Clone S258-5 (ASM10242). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Clcn3 Monoclonal Antibody (ASM10242) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane, Endosome, Endosome membrane, Cytoplasmic Vesicle, Secretory Vesicle Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Clcn3 Antibody (D) Composite.
ASM10242-A680 Image
Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa CIcn3 protein using Mouse Anti-CIcn3 Monoclonal Antibody, Clone S258-5 (ASM10242). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-CIcn3 Monoclonal Antibody (ASM10242) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3 |
| Application: | WB |
Additional Text
Accession Number
NP_445815.2
Gene Name
CLCN3
Uniprot ID
P51792
Purification
Protein G purified
Gene ID
84360
Storage Note
-20C
ASM10242-A680 Datasheet
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