mGluR1/5 glutamate receptor Antibody
Catalogue Number: ASM10253-A680-ABG
ASM10253-A680 Image
ASM10253-A680 Image
ASM10253-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 glutamate receptor Monoclonal Antibody, Clone S75-3 (ASM10253). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-mGluR1/5 glutamate receptor Monoclonal Antibody (ASM10253) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X.
ASM10253-A680 Image
Western Blot analysis of Rat Brain Membrane showing detection of ~130 kDa mGluR5 protein using Mouse Anti-mGluR5 Monoclonal Antibody, Clone S75-33 (ASM10253). Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 10 µg. Block: 5% milk. Primary Antibody: Mouse Anti-mGluR5 Monoclonal Antibody (ASM10253) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~130 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG2a |
| Immunogen: | Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b |
| Application: | WB |
Additional Text
Gene Name
GRM5
Accession Number
NP_058708.1
Gene ID
24418
Uniprot ID
P31424
Purification
Protein G purified
Storage Note
-20C
ASM10253-A680 Datasheet
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