Pannexin-2 Antibody
Catalogue Number: ASM10254-A680-ABG
ASM10254-A680 Image
ASM10254-A680 Image
ASM10254-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Pannexin-2 Monoclonal Antibody, Clone S121A-31 (ASM10254). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Pannexin-2 Monoclonal Antibody (ASM10254) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X.
ASM10254-A680 Image
Western Blot analysis of Rat Brain Membrane showing detection of ~70 kDa Pannexin 2 protein using Mouse Anti-Pannexin 2 Monoclonal Antibody, Clone S121A-31 (ASM10254). Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Pannexin 2 Monoclonal Antibody (ASM10254) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~70 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | Fusion protein amino acids 11-664 (all but first 10 amino acids, encompassing extracellular and cytoplasmic domains) of rat Pannexin-2 |
| Application: | WB |
Additional Text
Gene Name
PANX2
Purification
Protein G purified
Accession Number
NP_955441.2
Uniprot ID
P60571
Storage Note
-20C
Gene ID
362979
ASM10254-A680 Datasheet
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