Notch1 Antibody
Catalogue Number: ASM10264-A700-ABG
ASM10264-A700 Image
ASM10264-A700 Image
ASM10264-A700 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Notch1 Monoclonal Antibody, Clone S253-32 (ASM10264). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody (ASM10264) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Notch1 Antibody (D) Composite.
ASM10264-A700 Image
Western Blot analysis of Rat Brain Membrane showing detection of ~270 kDa Notch1 protein using Mouse Anti-Notch1 Monoclonal Antibody, Clone S253-32 (ASM10264). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 µg). . Load: 10 µg. Block: 5% milk. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody (ASM10264) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~270 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1 |
| Application: | WB |
Additional Text
Gene Name
NOTCH1
Uniprot ID
Q01705
Accession Number
NP_032740.3
Gene ID
18128
Purification
Protein G purified
Storage Note
-20C
ASM10264-A700 Datasheet
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