GluN2A/NR2A Antibody
Catalogue Number: ASM10268-A680-ABG
ASM10268-A680 Image
ASM10268-A680 Image
ASM10268-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327A-38 (ASM10268). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody (ASM10268) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-GluN2A/NR2A Antibody. (C) Composite.
ASM10268-A680 Image
Western Blot analysis of Monkey COS cells transfected with GFP-tagged NR2A showing detection of ~170 kDa GluN2A/NR2A protein using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327A-38 (ASM10268). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NR2A. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody (ASM10268) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG2b |
| Immunogen: | Fusion protein amino acids 75-325 (extracellular N-terminus) of rat GluN2A/NR2A |
| Application: | WB |
Additional Text
Gene Name
GRIN2A
Accession Number
NP_036705.3
Gene ID
24409
Uniprot ID
Q00959
Purification
Protein G purified
Storage Note
-20C
ASM10268-A680 Datasheet
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