Synaptotagmin-12 Antibody
Catalogue Number: ASM10271-A680-ABG
ASM10271-A680 Image
ASM10271-A680 Image
ASM10271-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Synaptotagmin-12 Monoclonal Antibody, Clone S277-7 (ASM10271). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-12 Monoclonal Antibody (ASM10271) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Synaptotagmin-12 Antibody (D) Composite.
ASM10271-A680 Image
Western Blot analysis of Monkey COS cells transfected with GFP-tagged Synaptotagmin showing detection of ~45 kDa Synaptotagmin-12 protein using Mouse Anti-Synaptotagmin-12 Monoclonal Antibody, Clone S277-7 (ASM10271). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Synaptotagmin. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Synaptotagmin-12 Monoclonal Antibody (ASM10271) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~45 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | Fusion protein amino acids 168-255 (Cytoplasmic C2A domain) of mouse Synaptotagmin-12 |
| Application: | WB |
Additional Text
Gene Name
SYT12
Purification
Protein G purified
Accession Number
NP_598925.1
Uniprot ID
Q920N7
Storage Note
-20C
Gene ID
171180
ASM10271-A680 Datasheet
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