ATF4 Antibody
Catalogue Number: ASM10281-A680-ABG
ASM10281-A680 Image
ASM10281-A680 Image
ASM10281-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ATF4 Monoclonal Antibody, Clone S360A-24 (ASM10281). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody (ASM10281) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATF4 Antibody (D) Composite.
ASM10281-A680 Image
Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4 protein using Mouse Anti-ATF4 Monoclonal Antibody, Clone S360A-24 (ASM10281). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody (ASM10281) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG2a |
| Immunogen: | Fusion protein amino acids 25-327 of human ATF4. 86% identical to rat, and 85% identical to mouse. <50% identity with ATF5. |
| Application: | WB |
Additional Text
Gene Name
ATF4
Gene ID
468
Uniprot ID
P18848
Accession Number
NP_001666.2
Purification
Protein G purified
Storage Note
-20C
ASM10281-A680 Datasheet
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