GRK (pan) Antibody
Catalogue Number: ASM10283-A680-ABG
ASM10283-A680 Image
ASM10283-A680 Image
ASM10283-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRK (pan) Monoclonal Antibody, Clone S145-20 (ASM10283). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRK (pan) Monoclonal Antibody (ASM10283) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GRK (pan) Antibody (D) Composite.
ASM10283-A680 Image
Western Blot analysis of Monkey COS cells transfected with untagged GRK6 showing detection of ~65 kDa GRK (pan) protein using Mouse Anti-GRK (pan) Monoclonal Antibody, Clone S145-20 (ASM10283). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with untagged GRK6. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GRK (pan) Monoclonal Antibody (ASM10283) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~65 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | Fusion protein amino acids 381-590 (C-terminus) of mouse GRK5. 91% identical to human, and 99% identical to rat. >55% identity with GRK6 and GRK4. >40% identity with GRK7 and RK. |
| Application: | WB |
Additional Text
Gene Name
GRK5
Purification
Protein G purified
Accession Number
NP_061357.3
Uniprot ID
Q8VEB1
Gene ID
14773
Storage Note
-20C
ASM10283-A680 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations