Protocadherin Gamma B2 Antibody
Catalogue Number: ASM10286-A680-ABG
ASM10286-A680 Image
ASM10286-A680 Image
ASM10286-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma B2 Monoclonal Antibody, Clone S148-30 (ASM10286). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma B2 Monoclonal Antibody (ASM10286) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoskeleton. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Protocadherin Gamma B2 Antibody (D) Composite.
ASM10286-A680 Image
Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-B2 showing detection of ~100 kDa Protocadherin Gamma B2 protein using Mouse Anti-Protocadherin Gamma B2 Monoclonal Antibody, Clone S148-30 (ASM10286). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-B2. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma B2 Monoclonal Antibody (ASM10286) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | Fusion protein amino acids 719-804 (variable cytoplasmic domain) of mouse Gamma- Protocadherin Gamma B2. Human: 68% identity (60/88 amino acids identical) >60% identity with other Gamma-protocadherin-B proteins |
| Application: | WB |
Additional Text
Gene Name
PCDHGB2
Purification
Protein G purified
Accession Number
NP_291053.1
Uniprot ID
Q91XX7
Gene ID
93700
Storage Note
-20C
ASM10286-A680 Datasheet
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