LGI1 Antibody
Catalogue Number: ASM10293-A565-ABG
ASM10293-A565 Image
ASM10293-A565 Image
ASM10293-A565 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LGI1 Monoclonal Antibody, Clone S283-7 (ASM10293). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LGI1 Monoclonal Antibody (ASM10293) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Golgi Apparatus, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) LGI1 Antibody (D) Composite.
ASM10293-A565 Image
Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa LGI1 protein using Mouse Anti-LGI1 Monoclonal Antibody, Clone S283-7 (ASM10293). Load: 10 µg. Primary Antibody: Mouse Anti-LGI1 Monoclonal Antibody (ASM10293) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~60 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG2a |
| Immunogen: | Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4. |
| Application: | WB |
Additional Text
Purification
Protein G purified
Uniprot ID
Q9JIA1.1
Accession Number
NP_064674.1
Storage Note
-20C
ASM10293-A565 Datasheet
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