NrCAM Antibody
Catalogue Number: ASM10294-A680-ABG
ASM10294-A680 Image
ASM10294-A680 Image
ASM10294-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NrCAM Monoclonal Antibody, Clone S364-51 (ASM10294). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NrCAM Monoclonal Antibody (ASM10294) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NrCAM Antibody (D) Composite.
ASM10294-A680 Image
Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa NrCam protein using Mouse Anti-NrCam Monoclonal Antibody, Clone S364-51 (ASM10294). Load: 10 µg. Primary Antibody: Mouse Anti-NrCam Monoclonal Antibody (ASM10294) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~160 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG2a |
| Immunogen: | Fusion protein amino acids 30-845 (extracellular domain) of mouse NrCAM. Rat: 96% identity (795/822 amino acids identical). Human: 91% identity (753/822 amino acids identical) ~50% identity with Neurofascin. |
| Application: | WB |
Additional Text
Gene Name
NRCAM
Purification
Protein G purified
Uniprot ID
Q810U4
Accession Number
NP_001139503.1
Gene ID
319504
Storage Note
-20C
ASM10294-A680 Datasheet
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