Neuroligin 1 Antibody
Catalogue Number: ASM10295-A680-ABG
ASM10295-A680 Image
ASM10295-A680 Image
ASM10295-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 1 Monoclonal Antibody, Clone S97A-31 (ASM10295). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Neuroligin 1 Monoclonal Antibody (ASM10295) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Neuroligin 1 Antibody (D) Composite.
ASM10295-A680 Image
Western Blot analysis of Mouse Brain Membrane showing detection of ~120 kDa Neuroligin 1 protein using Mouse Anti-Neuroligin 1 Monoclonal Antibody, Clone S97A-31 (ASM10295). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 1 Monoclonal Antibody (ASM10295) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~120 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | Fusion protein amino acids 718-843 (cytoplasmic C-terminus) of rat Neuroligin-1. Mouse: 99% identity (125/126 amino acids identical). Human: 99% identity (125/126 amino acids identical) >40% identity with Neuroligin-2 and -3. |
| Application: | WB |
Additional Text
Gene Name
Nlgn1
Uniprot ID
Q62765
Purification
Protein G purified
Accession Number
NP_446320.1.
Gene ID
116647
Storage Note
-20C
ASM10295-A680 Datasheet
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