INPP5F Antibody
Catalogue Number: ASM10296-A680-ABG
ASM10296-A680 Image
ASM10296-A680 Image
ASM10296-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-INPP5F Monoclonal Antibody, Clone S166A-26 (ASM10296). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-INPP5F Monoclonal Antibody (ASM10296) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Endosome, Endosome Membrane, Membrane, Golgi Apparatus, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) INPP5F Antibody (D) Composite.
ASM10296-A680 Image
Western Blot analysis of Monkey COS cells transfected with GFP-tagged OCRL showing detection of ~100 kDa INPP5F protein using Mouse Anti-INPP5F Monoclonal Antibody, Clone S166A-26 (ASM10296). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged OCRL. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-INPP5F Monoclonal Antibody (ASM10296) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | Fusion protein amino acids 1-901 (full-length) of human INPP5F. Rat: 93% identity (845/904 amino acids identical). Mouse: 91% identity (824/901 amino acids identical) ~50% identity with INPP5b. |
| Application: | WB |
Additional Text
Gene Name
OCRL
Gene ID
4952
Uniprot ID
Q01968
Purification
Protein G purified
Accession Number
NP_000267.2
Storage Note
-20C
ASM10296-A680 Datasheet
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