Neuroligin 3 Antibody
Catalogue Number: ASM10303-A680-ABG
ASM10303-A680 Image
ASM10303-A680 Image
ASM10303-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone S110-29 (ASM10303). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody (ASM10303) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction, Synapse . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Neuroligin 3 Antibody (D) Composite.
ASM10303-A680 Image
Western Blot analysis of Mouse Brain Membrane showing detection of ~110 kDa Neuroligin 3 protein using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone S110-29 (ASM10303). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody (ASM10303) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | Fusion protein amino acids 730-848 (intracellular C-terminus) of rat Neuroligin-3. Mouse: 99% identity (118/119 amino acids identical). Human: 98% identity (116/119 amino acids identical) ~60% identity with Neuroligin-1. ~40% identity with Neuroligin-2. |
| Application: | WB |
Additional Text
Gene Name
NLGN3
Gene ID
171297
Uniprot ID
Q62889
Purification
Protein G purified
Accession Number
AAA97871
Storage Note
-20C
ASM10303-A680 Datasheet
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