HSF1 Antibody
Catalogue Number: ASM10309-A655-ABG
ASM10309-A655 Image
ASM10309-A655 Image
ASM10309-A655 Image
ASM10309-A655 Image
ASM10309-A655 Image
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (ASM10309). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10309) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
ASM10309-A655 Image
Immunohistochemistry analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (ASM10309). Tissue: Breast carcinoma. Species: Human. Fixation: 10% Formalin Solution for 20 hours at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10309) at 1:2000 for 40 min. Secondary Antibody: Dako labeled Polymer HRP Anti-rat IgG, DAB Chromogen (brown) (Dako Envision+ System) for 30 min at RT. Counterstain: Mayer's Hematoxylin (purple/blue) nuclear stain for 1 minute at RT. Localization: Nuclear. Magnification: 100X. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.
ASM10309-A655 Image
Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of ~85 and 95 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (ASM10309). Lane 1: Molecular Weight Ladder. Lane 2: A431 cell lysates. Lane 3: HEK293 cell lysates. Load: 20 µg. Block: 5% milk + TBST 1hr at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10309) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rat: HRP at 1:1000 for 60 min at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~85 and 95 kDa.
ASM10309-A655 Image
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (ASM10309). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10309) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439 |
| Application: | WB, IHC |
Additional Text
Gene Name
HSF1
Gene ID
15499
Uniprot ID
P38532
Purification
Protein G purified
Accession Number
NP_032322.1
Storage Note
-20C
ASM10309-A655 Datasheet
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