beta 4 Spectrin Antibody
Catalogue Number: ASM10316-A565-ABG
ASM10316-A565 Image
ASM10316-A565 Image
ASM10316-A565 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-beta 4 Spectrin Monoclonal Antibody, Clone S393-2 (ASM10316). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody (ASM10316) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) beta 4 Spectrin Antibody (D) Composite.
ASM10316-A565 Image
Western Blot analysis of COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2 (ASM10316). Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody (ASM10316) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin |
| Application: | WB |
Additional Text
Uniprot ID
Q9H254
Gene ID
57731
Gene Name
SPTBN4
Purification
Protein G purified
Accession Number
NP_066022.2
Storage Note
-20C
ASM10316-A565 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations