GABA A Receptor Epsilon Antibody
Catalogue Number: ASM10325-A680-ABG
ASM10325-A680 Image
ASM10325-A680 Image
ASM10325-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Epsilon Monoclonal Antibody, Clone S413-67 (ASM10325). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Epsilon Monoclonal Antibody (ASM10325) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABA-A Receptor Epsilon Antibody (D) Composite.
ASM10325-A680 Image
Western Blot analysis of Rat Brain showing detection of ~60 kDa GABA A Receptor Epsilon protein using Mouse Anti-GABA A Receptor Epsilon Monoclonal Antibody, Clone S413-67 (ASM10325). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 10 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA A Receptor Epsilon Monoclonal Antibody (ASM10325) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG2a |
| Immunogen: | Fusion protein amino acids 27-253 (Extracellular N-terminus) of human GABA A Receptor Epsilon |
| Application: | WB |
Additional Text
Accession Number
NP_004952.2
Gene ID
2564
Gene Name
GABRE
Uniprot ID
P78334
Purification
Protein G purified
Storage Note
-20C
ASM10325-A680 Datasheet
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