Hexanoyl-Lysine adduct Antibody
Catalogue Number: ASM10337-A390-ABG
ASM10337-A390 Image
ASM10337-A390 Image
ASM10337-A390 Image
ASM10337-A390 Image
ASM10337-A390 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8 (ASM10337). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody (ASM10337) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
ASM10337-A390 Image
Western Blot analysis of Hexanoyl Lysine-BSA Conjugate showing detection of 67 kDa Hexanoyl-Lysine adduct-BSA using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8 (ASM10337). Lane 1: Molecular Weight Ladder (MW). Lane 2: Hexanoyl Lysine-BSA. Lane 3: BSA. Load: 0.5 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody (ASM10337) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
ASM10337-A390 Image
Flow Cytometry analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8 (ASM10337). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody (ASM10337) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT.
ASM10337-A390 Image
Western Blot analysis of Human Cervical Cancer cell line (HeLa) showing detection of Hexanoyl-Lysine adduct-BSA using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8 (ASM10337). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody (ASM10337) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH). |
| Application: | FC, WB |
Additional Text
Purification
Protein G purified
Storage Note
-20C
ASM10337-A390 Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations