GRP94 Antibody
Catalogue Number: ASM10352-A700-ABG
ASM10352-A700 Image
ASM10352-A700 Image
ASM10352-A700 Image
ASM10352-A700 Image
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP94 Polyclonal Antibody (ASM10352). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP94 Polyclonal Antibody (ASM10352) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
ASM10352-A700 Image
Western blot analysis of Rat brain cell lysates showing detection of ~ 94-100 kDa GRP94 protein using Rabbit Anti-GRP94 Polyclonal Antibody (ASM10352). Lane 1: MW ladder. Lane 2: Anti-GRP94 (1:250). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GRP94 Polyclonal Antibody (ASM10352) at 1:250 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP antibody at 1:50-1:100 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~ 94-100 kDa. Other Band(s): ~50, ~75 kDa.
ASM10352-A700 Image
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP94 Polyclonal Antibody (ASM10352). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP94 Polyclonal Antibody (ASM10352) at 1:120 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | Synthetic peptide corresponding to the sequence near the C-terminus of mouse GRP94 |
| Application: | WB |
Additional Text
Gene Name
HSP90B1
Uniprot ID
P08113
Accession Number
NP_035761.1
Purification
Affinity Purified
Gene ID
22027
Storage Note
-20C
ASM10352-A700 Datasheet
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