Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-KDEL Polyclonal Antibody (ASM10368). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-KDEL Polyclonal Antibody (ASM10368) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-KDEL Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.
ASM10368-A700 Image
Western blot analysis of Human Cell line lysates showing detection of KDEL protein using Rabbit Anti-KDEL Polyclonal Antibody (ASM10368). Primary Antibody: Rabbit Anti-KDEL Polyclonal Antibody (ASM10368) at 1:1000, 1:500.
ASM10368-A700 Image
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-KDEL Polyclonal Antibody (ASM10368). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-KDEL Polyclonal Antibody (ASM10368) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-KDEL Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.