Calnexin-CT Antibody

Catalogue Number: ASM10440-A655-ABG

ASM10440-A655 Image

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440) at 1:80 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Melanosome. Magnification: 100x. Heat Shocked at 42°C for 1h.

ASM10440-A655 Image

Western blot analysis of Rat tissue mix showing detection of Calnexin-CT protein using Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

ASM10440-A655 Image

Immunohistochemistry analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Hair Follicles, Basal cells in epidermis, and second layer of epidermis.

ASM10440-A655 Image

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Nuclear staining, cytoplasmic staining.

ASM10440-A655 Image

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (ASM10440) at 1:80 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Calnexin-CT Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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Manufacturer:	Abcepta
Type:	Primary Antibody
Shipping Condition:	Blue Ice
Unit(s):	each
Host name:
Clone:
Isotype:
Immunogen:	Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Application:	WB, IHC