Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSPB2 Polyclonal Antibody (ASM10454). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HSPB2 Polyclonal Antibody (ASM10454) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSPB2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
ASM10454-A700 Image
Western blot analysis of Rat tissue mix showing detection of HSPB2 protein using Rabbit Anti-HSPB2 Polyclonal Antibody (ASM10454). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSPB2 Polyclonal Antibody (ASM10454) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.