Beclin 1 Antibody
Catalogue Number: ASM10490-A680-ABG
ASM10490-A680 Image
ASM10490-A680 Image
ASM10490-A680 Image
ASM10490-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 1 Polyclonal Antibody (ASM10490). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody (ASM10490) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Golgi apparatus, Dendrites and Cell bodies of cerebellar Purkinje cells. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 1 Antibody (D) Composite.
ASM10490-A680 Image
Western blot analysis of Human HeLa cell lysates showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody (ASM10490). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody (ASM10490) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.
ASM10490-A680 Image
Western blot analysis of Human 293T showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody (ASM10490). Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody (ASM10490) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | Synthetic peptide from the C-terminus of human Beclin 1 |
| Application: | WB |
Additional Text
Gene Name
BECN1
Gene ID
8678
Accession Number
NP_001300927.1
Uniprot ID
Q14457
Purification
Affinity Purified
Storage Note
-20C
ASM10490-A680 Datasheet
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