Beclin 2 Antibody
Catalogue Number: ASM10491-A700-ABG
ASM10491-A700 Image
ASM10491-A700 Image
ASM10491-A700 Image
ASM10491-A700 Image
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10491). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10491) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 2 Antibody (D) Composite.
ASM10491-A700 Image
Western blot analysis of Human 293Trap cell lysates showing detection of ~48.1 kDa Beclin2 protein using Rabbit Anti-Beclin2 Polyclonal Antibody (ASM10491). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin2 Polyclonal Antibody (ASM10491) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.1 kDa.
ASM10491-A700 Image
Western blot analysis of Rat Liver showing detection of ~48kDa Beclin-2 protein using Rabbit Anti-Beclin-2 Polyclonal Antibody (ASM10491). Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin-2 Polyclonal Antibody (ASM10491) at 1:200 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~48kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | Synthetic peptide from the mid-protein of human Beclin 2 |
| Application: | WB |
Additional Text
Accession Number
NP_001277622.1
Gene ID
441925
Uniprot ID
A8MW95
Purification
Affinity Purified
Gene Name
BECN2
Storage Note
-20C
ASM10491-A700 Datasheet
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