ATG12 Antibody
Catalogue Number: ASM10497-A680-ABG
ASM10497-A680 Image
ASM10497-A680 Image
ASM10497-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG12 Polyclonal Antibody (ASM10497). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody (ASM10497) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG12 Antibody (D) Composite.
ASM10497-A680 Image
Western blot analysis of Human HeLa cell lysates showing detection of ~15kDa ATG12 protein using Rabbit Anti-ATG12 Polyclonal Antibody (ASM10497). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody (ASM10497) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~15kDa. Other Band(s): ATG12-ATG5 Complex.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | Synthetic peptide from the C-terminal of human ATG12 |
| Application: | WB |
Additional Text
Gene Name
ATG12
Uniprot ID
O94817
Accession Number
NP_001264712.1
Gene ID
9140
Purification
Affinity Purified
Storage Note
-20C
ASM10497-A680 Datasheet
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