ATG4A Antibody
Catalogue Number: ASM10510-A680-ABG
ASM10510-A680 Image
ASM10510-A680 Image
ASM10510-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4A Polyclonal Antibody (ASM10510). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody (ASM10510) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4A Antibody (D) Composite.
ASM10510-A680 Image
Western blot analysis of Human HeLa cell lysates showing detection of 45.3 kda ATG4A protein using Rabbit Anti-ATG4A Polyclonal Antibody (ASM10510). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody (ASM10510) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 45.3 kda.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | Synthetic peptide from the N-terminal of Human ATG4A (aa. 58-70) |
| Application: | WB |
Additional Text
Gene ID
115201
Gene Name
ATG4A
Uniprot ID
Q8WYN0
Accession Number
NP_001308216.1
Purification
Affinity Purified
Storage Note
-20C
ASM10510-A680 Datasheet
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