ATG4B Antibody
Catalogue Number: ASM10512-A680-ABG
ASM10512-A680 Image
ASM10512-A680 Image
ASM10512-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4B Polyclonal Antibody (ASM10512). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody (ASM10512) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4B Antibody (D) Composite.
ASM10512-A680 Image
Western blot analysis of Mouse Brain cell lysates showing detection of 44.3 kDa ATG4B protein using Rabbit Anti-ATG4B Polyclonal Antibody (ASM10512). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody (ASM10512) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 44.3 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | Synthetic peptide from the N-terminal of Human ATG4B (aa. 62-73) |
| Application: | WB |
Additional Text
Gene ID
23192
Gene Name
ATG4B
Uniprot ID
Q9Y4P1
Accession Number
NP_037457.3
Purification
Affinity Purified
Storage Note
-20C
ASM10512-A680 Datasheet
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