ATG4C Antibody
Catalogue Number: ASM10514-A680-ABG
ASM10514-A680 Image
ASM10514-A680 Image
ASM10514-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody (ASM10514). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody (ASM10514) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG4C Antibody. (D) Composite.
ASM10514-A680 Image
Western blot analysis of Human HEK293T cell lysates showing detection of 53 kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody (ASM10514). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HEK293T cell lysates. Load: 15 µg . Block: 2% GE Ashermsham in 1X TBST. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody (ASM10514) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 minutes in RT. Predicted/Observed Size: 53 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | Synthetic peptide from the C-terminal of Human ATG4C (aa. 296-306) |
| Application: | WB |
Additional Text
Gene ID
84938
Gene Name
ATG4C
Uniprot ID
Q96DT6
Accession Number
NP_116241.2
Purification
Affinity Purified
Storage Note
-20C
ASM10514-A680 Datasheet
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