SQSTM1 Antibody
Catalogue Number: ASM10517-A700-ABG
ASM10517-A700 Image
ASM10517-A700 Image
ASM10517-A700 Image
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SQSTM1 Polyclonal Antibody (ASM10517). Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-SQSTM1 Polyclonal Antibody (ASM10517) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Late Endosome, Lysosome, Cytoplasmic Vesicle, Autophagosome, Nucleus, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SQSTM1 Antibody (D) Composite.
ASM10517-A700 Image
Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 47.7 kDa SQSTM1 protein using Rabbit Anti-SQSTM1 Polyclonal Antibody (ASM10517). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SQSTM1 Polyclonal Antibody (ASM10517) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 47.7 kDa. Other Band(s): ~65 kDa in all lysates.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | Synthetic peptide from the N-terminal to mid of Human SQSTM1 (aa. 195-205) |
| Application: | WB |
Additional Text
Gene Name
SQSTM1
Uniprot ID
Q13501
Gene ID
8878
Accession Number
NP_001135770.1
Purification
Affinity Purified
Storage Note
-20C
ASM10517-A700 Datasheet
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