AMBRA1 Antibody
Catalogue Number: ASM10523-A700-ABG
ASM10523-A700 Image
ASM10523-A700 Image
ASM10523-A700 Image
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AMBRA1 Polyclonal Antibody (ASM10523). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody (ASM10523) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) AMBRA1 Antibody (D) Composite.
ASM10523-A700 Image
Western blot analysis of Rat Brain cell lysates showing detection of 142.5 kDa AMBRA1 protein using Rabbit Anti-AMBRA1 Polyclonal Antibody (ASM10523). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody (ASM10523) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 142.5 kDa. Other Band(s): 50 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | Synthetic peptide from the C-terminal of Human AMBRA1 (aa. 202-212) |
| Application: | WB |
Additional Text
Gene Name
AMBRA1
Gene ID
55626
Uniprot ID
Q9C0C7
Accession Number
NP_001254711.1
Purification
Affinity Purified
Storage Note
-20C
ASM10523-A700 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations