PI 3 Kinase Class 3 antibody
Catalogue Number: ASM10533-A655-ABG
ASM10533-A655 Image
ASM10533-A655 Image
ASM10533-A655 Image
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (ASM10533). Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (ASM10533) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite.
ASM10533-A655 Image
Western blot analysis of Rat, Mouse Liver cell lysates showing detection of ~101.5 kDa PI 3 Kinase Class 3 protein using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (ASM10533). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Lane 3: Mouse Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (ASM10533) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~101.5 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | Synthetic peptide from the N-terminal of Human PI 3 Kinase Class 3 |
| Application: | WB |
Additional Text
Gene Name
PIK3C3
Gene ID
5289
Uniprot ID
Q8NEB9
Accession Number
NP_002638.2
Purification
Affinity Purified
Storage Note
-20C
ASM10533-A655 Datasheet
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