IRGM Antibody
Catalogue Number: ASM10541-A655-ABG
ASM10541-A655 Image
ASM10541-A655 Image
ASM10541-A655 Image
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody (ASM10541). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody (ASM10541) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.
ASM10541-A655 Image
Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of ~20.1 kDa IRGM protein using Rabbit Anti-IRGM Polyclonal Antibody (ASM10541). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody (ASM10541) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~20.1 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | Synthetic peptide from the N-terminal of Human IRGM |
| Application: | WB |
Additional Text
Gene ID
345611
Gene Name
IRGM
Uniprot ID
A1A4Y4
Accession Number
NP_001139277.1
Purification
Affinity Purified
Storage Note
-20C
ASM10541-A655 Datasheet
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