beta Actin Antibody
Catalogue Number: ASM10547-A390-ABG
ASM10547-A390 Image
ASM10547-A390 Image
ASM10547-A390 Image
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beta Actin Polyclonal Antibody (ASM10547). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beta Actin Polyclonal Antibody (ASM10547) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm, Cytoskeleton. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beta Actin Antibody (D) Composite.
ASM10547-A390 Image
Western blot analysis of Human HeLa and 293T cell lysates showing detection of ~41.7kDa beta Actin protein using Rabbit Anti-beta Actin Polyclonal Antibody (ASM10547). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-beta Actin Polyclonal Antibody (ASM10547) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~41.7kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | Synthetic peptide from the mid-protein range of human beta Actin |
| Application: | WB |
Additional Text
Gene Name
ACTB
Gene ID
60
Accession Number
NP_001092.1
Uniprot ID
P60709
Purification
Affinity Purified
Storage Note
-20C
ASM10547-A390 Datasheet
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