Anti-Human IgG (H&L) (Alkaline Phosphatase Conjugated) Secondary Antibody
Catalogue Number: ASR2176-ABG
ASR2176 Image
ASR2176 Image
ASR2176 Image
Western Blot of Alkaline Phosphatase conjugated Goat anti-Human IgG antibody. Lane 1: Human IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase Human secondary antibody at 1:1,000 o/n at 4°C. Block: MB-070 for 30 min at RT. Reaction visualized using alkaline phosphatase for 30 seconds at RT. Predicted/Observed size: 50kDa, 25kDa for Human IgG.
ASR2176 Image
Dot Blot of Alkaline Phosphatase conjugated Goat anti-Human IgG antibody. Antigen: Human IgG. Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng. Primary antibody: none. Secondary antibody: Alkaline Phosphatase conjugated Goat anti-Human IgG antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.
| Manufacturer: | Abcepta |
| Shelf Life: | 3 weeks |
| Preservative: | 0.01% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Secondary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Human IgG whole molecule |
| Application: | DB |
Description
Description: Anti-Human IgG (H&L) detects human Immunoglobulin G (IgG) antibody isotype. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation.[1] IgG molecules are synthesized and secreted by plasma B cells.
Additional Text
Concentration
0.5 mg/ml
Antibody Clonality
Polyclonal
Application Notes
ELISA 1:4,500;Western Blot 1:500-1:2,500;Immunochemistry 1:200-1:1,000
Storage Note
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Short Description
Goat Polyclonal, Alkaline Phosphatase (Calf Intestine)
ASR2176 Datasheet
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