Anti-RABBIT IgG (H&L) (Alkaline Phosphatase Conjugated) Secondary Antibody
Catalogue Number: ASR2214-ABG
ASR2214 Image
ASR2214 Image
ASR2214 Image
ASR2214 Image
Western Blot of Goat anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated. Lane 1: Rabbit IgG. Lane 2: None. Load: 100 ng per lane. Primary Antibody: None. Secondary antibody: Alkaline Phosphatase goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 kDa for Rabbit IgG. Other Band(s): None.
ASR2214 Image
Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody . Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule . Lane 2: Rabbit IgG F(ab) Fragment . Lane 3: Rabbit IgG F(c) Fragment . Lane 4: Rabbit IgM Whole Molecule . Lane 5: Normal Rabbit Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
ASR2214 Image
Dot Blot of Rabbit IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Rabbit IgG. Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng. Primary antibody: none. Secondary antibody: Rabbit IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.
| Manufacturer: | Abcepta |
| Shelf Life: | 3 weeks |
| Preservative: | 0.1% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Secondary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat. |
| Application: | WB, DB |
Description
Description: Anti-Rabbit IgG alkaline phosphatase conjugated antibody generated in goat detects specifically rabbit IgG. This secondary alkaline phosphatase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Additional Text
Concentration
0.5 mg/ml
Antibody Clonality
Polyclonal
Application Notes
ELISA 1:30,000;Western Blot 1:1,000-1:4,000;Immunochemistry 1:200-1:1,000
Storage Note
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Short Description
Goat Polyclonal, Alkaline Phosphatase (Calf Intestine)
ASR2214 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations