Anti-Rabbit IgG (H&L) (Alkaline Phosphatase Conjugated) Secondary Antibody
Catalogue Number: ASR2219-ABG
ASR2219 Image
ASR2219 Image
ASR2219 Image
ASR2219 Image
Western Blot of Anti-Rabbit IgG (H&L) (SHEEP) Antibody (Min X Hu, Gt, Ms Serum Proteins) . Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule . Lane 2: Rabbit IgG F(ab) Fragment . Lane 3: Rabbit IgG F(c) Fragment . Lane 4: Rabbit IgM Whole Molecule . Lane 5: Normal Rabbit Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (SHEEP) Antibody (Min X Hu, Gt, Ms Serum Proteins) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Sheep IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
ASR2219 Image
Western blot of Alkaline Phosphatase conjugated Sheep Anti-Rabbit IgG secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Alkaline Phosphatase sheep secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.
ASR2219 Image
Dot Blot of Sheep anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Rabbit IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Sheep anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 1 HR at RT.
| Manufacturer: | Abcepta |
| Shelf Life: | 3 weeks |
| Preservative: | 0.01% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Secondary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Sheep |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Rabbit IgG whole molecule |
| Application: | WB, DB |
Description
Description: Rabbit IgG antibody detects rabbit IgG whole molecule heavy and light chain components. Anti-Rabbit IgG antibody is ideal for researchers using immunoassays with rabbit primary antibody reagents.
Additional Text
Concentration
0.5 mg/ml
Antibody Clonality
Polyclonal
Application Notes
ELISA 1:15,000;Western Blot 1:500-1:2,000;Immunochemistry 1:200-1:1,000
Storage Note
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Short Description
Sheep Polyclonal, Alkaline Phosphatase (Calf Intestine)
ASR2219 Datasheet
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