Anti-Rat IgG (H&L) (Alkaline Phosphatase Conjugated) Secondary Antibody
Catalogue Number: ASR2802-ABG
ASR2802 Image
ASR2802 Image
Western Blot of Anti-Rat IgG (H&L) (CHICKEN) Antibody . Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule . Lane 2: Rat IgG F(c) Fragment . Lane 3: Rat IgG F(ab) Fragment . Lane 4: Rat IgM Whole Molecule . Lane 5: Rat Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (CHICKEN) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Chicken IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and F(ab), 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
| Manufacturer: | Abcepta |
| Shelf Life: | 3 weeks |
| Preservative: | 0.01% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Secondary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Rat IgG whole molecule |
Additional Text
Concentration
1.2 mg/mL
Antibody Clonality
Polyclonal
Application Notes
ELISA 1:2,000-1:10,000;Western Blot 1:500-1:2,500;Immunohistochemistry 1:200-1:1,000
Storage Note
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Short Description
Chicken Polyclonal, Alkaline Phosphatase (Calf Intestine)
ASR2802 Datasheet
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