Anti-Rabbit IgG (H&L) (Peroxidase Conjugated) Pre-Adsorbed Secondary Antibody
Catalogue Number: ASR2961-ABG
ASR2961 Image
ASR2961 Image
ASR2961 Image
Western Blot of Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) . Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule . Lane 2: Rabbit IgG F(ab) Fragment . Lane 3: Rabbit IgG F(c) Fragment . Lane 4: Rabbit IgM Whole Molecule . Lane 5: Normal Rabbit Serum . All samples were reduced. Load: 50 ng of IgG, F(ab), F(c) and Serum, 25 ng of IgM. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) 1:7,500 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
ASR2961 Image
Western Blot of Peroxidase conjugated Donkey anti-Rabbit IgG antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase donkey secondary antibody at 1:1,000 for 60 min at RT Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Rabbit IgG. Other band(s): none.
| Manufacturer: | Abcepta |
| Preservative: | 0.01% Gentamicin sulfate |
| Physical state: | Lyophilized |
| Type: | Polyclonal Secondary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
| Host name: | Donkey |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in donkey. |
| Application: | WB |
Description
Description: Anti-Rabbit IgG peroxidase conjugated antibody generated in donkey detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Additional Text
Concentration
0.5 mg/ml
Antibody Clonality
Polyclonal
Application Notes
ELISA 1:10,000-1:150,000;Western Blot 1:1,000-1:5,000;Immunochemistry 1:500-1:2,500
Storage Note
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Short Description
Donkey Polyclonal, Peroxidase (Horseradish)
ASR2961 Datasheet
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