Anti-Mouse IgG1 (Alkaline Phosphatase Conjugated) Secondary Antibody
Catalogue Number: ASR3097-ABG
ASR3097 Image
ASR3097 Image
ASR3097 Image
Western Blot of Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated. Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4°C. Block: MB-070 for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Predicted/Observed size: 55 kDa for Mouse IgG1.
ASR3097 Image
Dot Blot of Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG1. Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1,000 for one hour at RT. Block: MB-070 for 60 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.
| Manufacturer: | Abcepta |
| Shelf Life: | 3 weeks |
| Preservative: | 0.01% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Secondary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG, IgG1 |
| Immunogen: | Mouse IgG1 heavy chain |
| Application: | DB |
Description
Description: Mouse IgG1 secondary antibody conjugated to Alkaline Phosphatase is available in a variety of formats. Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.
Additional Text
Concentration
0.5 mg/ml
Antibody Clonality
Polyclonal
Application Notes
ELISA 1:5,000;Western Blot 1:500-1:2,500;Immunochemistry 1:200-1:1,000
Storage Note
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Short Description
Rabbit Polyclonal, Alkaline Phosphatase (Calf Intestine)
ASR3097 Datasheet
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