Anti-Rabbit IgG (H&L) (Peroxidase Conjugated) Pre-Adsorbed Secondary Antibody
Catalogue Number: ASR3104-ABG
ASR3104 Image
ASR3104 Image
ASR3104 Image
Western blot of Peroxidase conjugated Rat Anti-Rabbit IgG (Pre-Adsorbed) secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rat secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.
ASR3104 Image
Western Blot of Anti-Rabbit IgG (H&L) (RAT) Antibody (Min X Hu, Gt, Ms Serum Proteins) . Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule . Lane 2: Rabbit IgG F(ab) Fragment . Lane 3: Rabbit IgG F(c) Fragment . Lane 4: Rabbit IgM Whole Molecule . Lane 5: Normal Rabbit Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (RAT) Antibody (Min X Hu, Gt, Ms Serum Proteins) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Rat IgG (RABBIT) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
| Manufacturer: | Abcepta |
| Preservative: | 0.01% Gentamicin sulfate |
| Physical state: | Lyophilized |
| Type: | Polyclonal Secondary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
| Host name: | Rat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Rabbit IgG whole molecule |
| Application: | WB |
Additional Text
Concentration
0.5 mg/ml
Antibody Clonality
Polyclonal
Application Notes
ELISA 1:10,000-1:50,000;Western Blot 1:1,000-1:10,000;Immunochemistry 1:500-1:2,500
Storage Note
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Short Description
Rat Polyclonal, Peroxidase (Horseradish)
ASR3104 Datasheet
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