Anti-Rat IgG (H&L) (Phycoerythrin Conjugated) Pre-Adsorbed Secondary Antibody
Catalogue Number: ASR3108-ABG
ASR3108 Image
ASR3108 Image
Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) . Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule . Lane 2: Rat IgG F(c) Fragment . Lane 3: Rat IgG F(ab) Fragment . Lane 4: Rat IgM Whole Molecule . Lane 5: Rat Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and F(ab), 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
| Manufacturer: | Abcepta |
| Shelf Life: | immediate use |
| Preservative: | 0.01% Sodium azide |
| Physical state: | Lyophilized |
| Type: | Polyclonal Secondary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 1 mL |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Anti-Rat IgG whole molecule was produced by repeated immunization with Rat IgG whole molecule in goat. |
Description
Description: Anti-Rat IgG whole molecule antibody generated in goat detects specifically Rat IgG whole molecule. This secondary antibody anti-Rat is ideal for investigators who routinely perform immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels.
Additional Text
Concentration
0.5 mg/ml
Antibody Clonality
Polyclonal
Application Notes
IF Microscopy 1:100-1:250;FlowCytometry 1:100-1:250;FLOW1:100-1:250
Storage Note
Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Short Description
Goat Polyclonal, R-Phycoerythrin (RPE)
ASR3108 Datasheet
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