F(ab')2 Anti-HUMAN IgG F(c) (Pre-adsorbed Phycoerythrin Conjugated) Secondary Antibody
Catalogue Number: ASR3188-ABG
ASR3188 Image
ASR3188 Image
Western blot of Phycoerythrin conjugated Goat F(ab')2 Anti-Human IgG F(c) (Pre-Adsorbed) secondary antibody. Lane 1: Human Fc. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Human IgG F(c). Other band(s): None.
| Manufacturer: | Abcepta |
| Shelf Life: | immediate use |
| Preservative: | 0.01% Sodium azide |
| Physical state: | Lyophilized |
| Type: | Polyclonal Secondary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 500 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Anti-Human IgG was produced by repeated immunization with Human IgG F(c) fragment in goat. |
Description
Description: F(ab')2 HUMAN IgG F(c) (Pre-Adsorbed) Phycoerythrin Conjugated Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 HUMAN IgG F(c) (Pre-Adsorbed) Phycoerythrin Conjugated Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
Additional Text
Concentration
0.5 mg/ml
Antibody Clonality
Polyclonal
Application Notes
IF Microscopy 1:100-1:250;FlowCytometry 1:100-1:250
Storage Note
Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Short Description
Goat Polyclonal, R-Phycoerythrin (RPE)
ASR3188 Datasheet
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