Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) (ATTO 425 Conjugated) Secondary Antibody
Catalogue Number: ASR3258-ABG
ASR3258 Image
ASR3258 Image
ASR3258 Image
ATTO 425 conjugated anti-Mouse IgG was used to demonstrate 2 color STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 µg/mL final concentration of anti-a-tubulin and detected with 0.2 µg/mL ATTO 425 conjugated anti-MOUSE IgG [GOAT] (610-151-121) secondary antibody (colored RED). Also shown in this 2-color STED image is Abcepta's Anti-HDAC-1 [RABBIT] detected with DyLight™488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody (colored GREEN). Image courtesy of Myriam Gastard, Leica Microsystems, USA.
ASR3258 Image
ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Joerg Reichwein, ATTO-TEC GmbH
| Manufacturer: | Abcepta |
| Preservative: | 0.01% Sodium azide |
| Physical state: | Lyophilized |
| Type: | Polyclonal Secondary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 500 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG, IgG1, IgG2a, IgG2b, IgG3 |
| Immunogen: | highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins |
| Application: | IF |
Description
Description: Mouse IgG subclass pan reactive Secondary Antibody is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Rabbit anti-mouse IgG antibody ATTO 425 is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Additional Text
Concentration
1 mg/ml
Antibody Clonality
Polyclonal
Application Notes
FLISA >1:20,000;IF Microscopy >1:5,000;Western Blot >1:10,000
Storage Note
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Short Description
Rabbit Polyclonal, ATTO 425
ASR3258 Datasheet
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