Anti-BETA GALACTOSIDASE (E.coli) (CHICKEN) Antibody
Catalogue Number: ASR4843-ABG
| Manufacturer: | Abcepta |
| Preservative: | 0.01% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Chicken |
| Clone: | |
| Isotype: | |
| Immunogen: | This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with full length native Beta Galactosidase isolated from E.coli |
Description
Description: Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti-beta Galactosidase Antibody is ideal for investigators involved in enzyme research.
Additional Text
Concentration
0.5 mg/ml
Uniprot ID
P00722
Gene ID
945006
Gene Name
lacZ
Antibody Clonality
Polyclonal
Storage Note
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Notes
Anti-Beta-Gal (CHICKEN) Antibody has been tested for use in ELISA and immunoblotting. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry, flow Cytometry, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity.
Purification
This Anti-Beta Galactosidase Antibody is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Beta Galactosidase [E.coli] and anti-Chicken Serum. Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.
Short Description
Beta Galactosidase Antibody
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