Anti-ATPase, Na+K+ (Canine Kidney) (CHICKEN) Antibody
Catalogue Number: ASR4852-ABG
| Manufacturer: | Abcepta |
| Preservative: | 0.01% Sodium azide |
| Physical state: | Lyophilized |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
| Host name: | Chicken |
| Clone: | |
| Isotype: | |
| Immunogen: | ATPase, Sodium, Potassium [Canine Kidney] |
Description
Description: Sodium/potassium-transporting ATPase subunit alpha-1 is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Additional Text
Concentration
10 mg/mL
Gene Name
ATP1A1
Antibody Clonality
Polyclonal
Uniprot ID
P50997
Gene ID
403992
Storage Note
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Notes
Anti-ATPase Na+ K+ has been assayed against 1.0 ug of ATPase, Sodium, Potassium in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit) code #603-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for this product.
Purification
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney]. Cross reactivity against ATPase, Sodium, Potassium from other tissues and species may occur but have not been specifically determined.
Short Description
ATPase Antibody
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