AKT2 Western Blot Kit

Catalogue Number: ASR5944-ABG

Manufacturer:	Abcepta
Type:	Protein Detection
Shipping Condition:	Dry Ice
Unit(s):	1 kit
Host name:	Rat
Clone:
Isotype:
Immunogen:

Overview

Description

Description: Rockland Immunochemicals' Chemiluminescent Western Blot Kit for AKT2 combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol chemiluminescent substrate for the detection of native and/or recombinant human AKT2 protein. AKT2 Western Blotting kit allows for the detection of endogenous protein levels of human AKT2 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT2. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT2 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT2 Western Blotting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.

Additional Text

Gene ID

208

Gene Name

AKT2

Uniprot ID

P31751

Antibody Clonality

Monoclonal

Storage Note

See kit insert for complete instructions.

Application Notes

Use Rockland Immunochemicals' Anti-AKT2 Chemiluminescent Kit for Western Blotting for detection of human AKT2 native and recombinant proteins by western blot. Expect a band approximately 56 kDa in size corresponding to AKT2 protein. This kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.

Purification

The kit is designed to detect both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT1 and AKT3 has not been determined. This kit contains sufficient substrate for up to 5 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.

Short Description

AKT2 Western Chemiluminescent Blotting Kit

Price

£407.00

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