APC Anti-Human CD4 (RPA-T4) Antibody

Catalogue Number: ATB10008-ABG

Manufacturer:	Abcepta
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	Leu-3, T4\|Leu-3, T4, CD4
Shipping Condition:	Blue Ice
Unit(s):	500 tests
Host name:	Mouse
Clone:	RPA-T4
Isotype:	IgG1, kappa
Immunogen:
Application:	FC

Overview
Datasheets

Description

Description: The RPA-T4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RPA-T4 antibody is widely used as a phenotypic marker for human CD4 expression, and is cross-reactive with Chimpanzee CD4. This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone OKT4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065).

Additional Text

Gene Name

CD4

Gene ID

920

Application Notes

This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.

Uniprot ID

P01730

Antibody Clonality

Monoclonal

Storage Note

2-8°C protected from light

ATB10008 Datasheet

Price

£336.00

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