In Vivo Ready™ Anti-Mouse CD4 (GK1.5) Antibody

Catalogue Number: ATB10141-ABG

Manufacturer:	Abcepta
Type:	Monoclonal Primary Antibody - Unconjugated
Alias:	L3T4, T4\|T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, CD4, Cd4
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	1 mg
Host name:	Rat
Clone:	GK1.5
Isotype:	IgG2b, kappa
Immunogen:
Application:	FC, IP, IHC-Fr, FA

Overview
Datasheets

Description

Description: The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The GK1.5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the GK1.5 antibody and an alternative antibody, Anti-Mouse CD4 clone RM4-5, will - ompete - for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the Anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells (Arora S et al. 2006. Infect. Immun. 74: 4339-4348). The GK1.5 antibody is also reported to be cross-reactive with Syrian hamster CD4.

Additional Text

Gene Name

CD4

Gene ID

12504

Application Notes

This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis. Citations are provided as a convenience to you - please consult Materials and Methods sections for additional details about the use of any product in these publications.

Uniprot ID

P06332

Antibody Clonality

Monoclonal

Storage Note

2-8°C

ATB10141 Datasheet

Price

£238.00

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