Catalogue Number: ATB10263-ABG
C57Bl/6 splenocytes were stained with 0.25 ug PerCP-Cy5.5 Anti-Mouse CD4 (ATB10263) (solid line) or 0.25 ug PerCP-Cy5.5 Rat IgG2a isotype control (dashed line).
C57Bl/6 splenocytes were stained with 0.25 ug PerCP-Cy5.5 Anti-Mouse CD4 (RM4-5) manufactured by Tonbo Biosciences (left panel) or eBioscience (right panel).
|Type:||Monoclonal Primary Antibody - Conjugated|
|Alias:||T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, CD4, Cd4|
|Shipping Condition:||Blue Ice|
|Unit(s):||25 ug, 100 ug|
|Isotype:||Rat IgG2a, kappa|
Description: The RM4-5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta2-domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will – ompete – for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.
This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system.
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.