violetFluor™ 450 Anti-Human/Mouse CD11b (M1/70) Antibody
Catalogue Number: ATB10347-ABG
ATB10347 Image
ATB10347 Image
ATB10347 Image
C57Bl/6 bone marrow cells were stained with 0.125 ug violetFluor 450 Anti-Hu/Mo CD11b (ATB10347) (solid line) or 0.125 ug violetFluor 450 Rat IgG2b isotype control (dashed line).
ATB10347 Image
C57Bl/6 bone marrow cells were stained with violetFluor 450 Anti-Human/Mouse CD11b (M1/70) manufactured by Tonbo Biosciences (left panel) or Horizon V450 Anti-Mouse/Human CD11b (M1/70) manufactured by BD Biosciences (right panel).
| Manufacturer: | Abcepta |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, ITGAM, CD11B, CR3A|Mac-1, integrin αM, CR3, ITGAM |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rat |
| Clone: | M1/70 |
| Isotype: | IgG2b, kappa |
| Immunogen: | |
| Application: | FC |
Description
Description: The M1/70 antibody reacts with human and mouse CD11b, also known as integrin alpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha M (CD11b) assembles with Integrin beta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.The M1/70 antibody is widely used as a marker for CD11b expression on mouse macrophages, granulocytes, neutrophils, and NK cells. The antibody is also reported to be cross-reactive for Rhesus macaque CD11b.
Additional Text
Gene Name
ITGAM
Gene ID
3684
Uniprot ID
P11215
Application Notes
This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. violetFluor™ 450 dye is excited by the violet (405 nm) laser and has a peak emission of 450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluor™ 450 can be used as an alternative for Pacific Blue®, BD Horizon™ V450 or eFluor® 450.
Antibody Clonality
Monoclonal
Storage Note
2-8°C protected from light
ATB10347 Datasheet
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